| A 3-day-old wild-type Xenopus larva was lightly fixed (2% paraformaldehyde overnight at 4°C) and then imaged with OCM. The movie consists of a single 3-D dataset volume-rendered at successively rotated angles and spliced together. The rotating image illustrates the difference in information conveyed from different perspectives of the same image. The pigmentation of the ectoderm, characteristic of wild-type larva, is clearly visible as the image begins to rotate. The movie pauses twice to show the two perspectives along the developing notochord. Visualization properties are slightly modified during the movie to illustrate the developing neurul structure. |